光气染毒造成小鼠肺脏的氧化损伤

The oxidant injury induced by phosgene of lung in mice

目的 研究小鼠光气染毒后不同时间对肺脏的氧化损伤作用。方法 4 0只雄性小鼠 ,随机分为 4组。正常对照组小鼠以空气为对照 ,染毒组小鼠给予 11.9mg/L剂量的光气 ,时间为5min ,分别染毒后 2h、4h、8h ,测定各组小鼠肺脏的湿干比和肺匀浆液的丙二醛 (MDA)含量、总超氧化物歧化酶 (T SOD)活力、还原型谷胱甘肽 (GSH)含量。结果 随着光气染毒时间的增加 ,小鼠肺脏的湿干比显著增加 (P <0 .0 5 ) ;染毒后 4h ,肺组织MDA含量和T SOD活力显著升高(P <0 .0 5 ) ,GSH含量在染毒后 2h显著降低 (P <0 .0 5 )。结论 光气染毒可引起小鼠肺水肿和肺脏氧化损伤。

Objective To study oxidative injury of lung in different time in mice after exposed to phosgene. Methods 40 mice were randomly divided into 4 groups. The mice in control group were exposed to air and the mice in case group were exposed to 11.9 mg·L -1 phosgene for 5 minutes, respectively. After 2 hours, 4 hours, 8 hours, all mice were killed and wet/dry radio, the content of malondialdehyde (MDA), reductive glutathion (GSH) and activities of the total superoxide dismutase (T-SOD) in lung were detected. Results With the time prolonged, wet/dry radio increased significantly ( P<0.05). After 4 hour, MDA content and T-SOD activity increased significantly ( P<0.05). GSH content decreased significantly in positive group, compared with negative group after 2 hours of phosgene exposure (P<0.05). Conclusions Phosgene might induce pulmonary edema and oxidative injury of lung in mice.