兔眼人工晶体植入术后TIMPs在晶体上皮细胞的表达及其意义

Expression and Significance of Tissue Inhibitors of Matrix Metalloprotenase on Lens Epithelial Cells in Rabbit Eyes with Intraocular Lens Implantation

目的 观察白内障囊外摘除及人工晶体植入 (ECC +IOL)术后基质金属蛋白酶抑制因子 (TIMPs)在晶体上皮细胞的表达 ,探讨TIMPs以ECCO +IOL术后后囊膜混浊的影响。方法 2 5只健康成年家兔 ,均一只眼行晶体囊外摘除及人工晶体植入术 ,另一只未手术眼作为对照组。每 5兔为一组 ,分别于术后 1、3、7、1 4、30d取出晶状体后囊膜 ,用RT -PCR检测各标本中的TIMPsmRNA的表达 ,对扩增产物用凝交成像系统进行定量分析 ,以TIMP/GAPDH的比值表示TIMPsmRNA的相对表达水平 ,并用羟脯氨酸试剂盒检测晶体囊膜羟脯氨酸量的变化。结果 在常晶体上皮细胞组织均有TIMP - 1、- 2、- 3和 - 4mNA的表达 ;术后第 1天 ,TIMP - 1、- 2、- 3和- 4mRNA均明显升高 ,术后第 7天 ,TIMP - 1、- 2和 - 3RNA的表达量达到最大 ,此后表达式量逐渐下降 ,术后第30天的表达量仍高于对照组 ,术后TIMP - 4mRNA则表现为下降 ;结论 白内障囊外摘除及人工晶体植入术后TIMPsmRNA在晶体上皮细胞的表达均明显升高 ,提示TIMPs可能是抑制白内障囊外摘除及人工晶体植入术后细胞外基质降解的主要因素 ,TIMPs可能是后囊膜混浊的形成和纤维化的重要原因之一。

Objective To study the expression of TIMPs mRNA in lens epithelial cells(LECs) in rabbit eyes after extracapsular cataract extraction(ECCE) with intraocular lens implantation(IOL), and to expound the effects of TIMPs on posterior capsule opacification.Methods 25 adult rabbits were studied. In each rabbit, one eye was undergone ECCE with IOL and the other eye was normal without any surgical intervention. The samples of lens capsules were collected on the postoperative 1,3,7,14 and 30 days respectively. The expression of TIMPs mRNA in the specimens was detected by using reverse transcription chain reaction(RT-PCR).In addition, the amount of collagen was analyzed by the assay kits of hydroxyproline.Results The expressions of TIMP -1 to -4 mDNA could be detected in the normal tissues. TIMP -1 to -4 mRNA in LECs with IOL was displayed a significant increase on the first day after operation and the amount of TIMPs -1 to -3 reached tiptop, after this, the expression of them declined gradually, but TIMPs mDNA were also higher than the normal tissues on 30 th day. TIMP -4 mRNA decreased gently from the fifth day to 30 th day after operation. The amount of hydroxyproline on postoperative 30 day was markedly higher than that of postoperative 1,3,7 and 14 days.Conclusion TIMPs mDNA on RLECs with ECCE+IOL is obviously high. TIMPs may be mainly due to decreased degradation of extracellular matrix after ECCE with IOL.This study suggests that TIMPs could be one of the major contributors to posterior capsular opacification and fibrosis.