不同培养基对小鼠小脑浦肯野细胞发育的影响

Development of Mouse Cerebellar Purkinje Cells in Different Media in vitro

目的 研究小鼠小脑浦肯野细胞的体外培养条件。方法 分别用有血清和无血清培养基培养小鼠小脑浦肯野细胞 ,应用免疫荧光染色和ABC法染色观察细胞形态 ,测定树突面积和胞体直径。结果 用无血清添加N3 的DMEM /F1 2培养基比有血清培养基培养的浦肯野细胞发育出的树突更多 ;在DMEM/F1 2培养基中加入T3 后浦肯野细胞的树突面积和胞体直径较大。结论 无血清培养基更有利于浦肯野细胞的维持培养 ;添加T3 的DMEM /F1 2 /N3 培养基有利于浦肯野细胞的发育。

Objective To find out a better culture medium for cultivating mouse cerebellar Purkinje cells.Methods Several culture media with serum or without serum were used to compare their survival rate and their dendritic differentiation of cerebellar Purkinje cells in a monolayer,mixed culture setting by immuno-fluorescence staining and ABC methods.Results Addition of a thyroid hormone,T 3,to DMEM/F-12,a serum-free medium resulted in a highly elaborate dentritic development of Purkinje cells.Conclusion Culture medium without serum is more suitable for maintaining its cultivation while an addition of T 3 to DMEM/f-12 is much better for the development of mouse cerebellar Purkinje cells.