Maspin蛋白在乳腺癌与癌旁组织中的表达及其意义

The Significance of Expression of Maspin in Breast Cancer and Lesions in the Neighborhood of Cancer

目的:探讨Maspin蛋白在乳腺癌与癌旁组织中的表达及其意义。方法:采用免疫组化SP法检测104例浸润性乳腺癌、26例癌旁原位癌成分、63例癌旁导管上皮增生性病变(不典型增生20例、单纯性增生43例)及10例正常乳腺组织中Maspin蛋白的表达情况,同时分析浸润性乳腺癌组织中Maspin蛋白表达与病理组织学分级、淋巴结转移及ER、PR、c-erbB-2、Cath-D、VEGF表达的关系。结果:正常乳腺上皮细胞Maspin蛋白阳性反应主要位于细胞核,所有正常乳腺上皮细胞核均呈高表达(++～+++,100%),乳腺上皮单纯性增生、不典型增生、原位癌和浸润性乳腺癌细胞核Maspin蛋白高表达率分别为76.7%、65.0%、69.2%,和49.0%。从正常乳腺上皮到癌旁不典型增生细胞核Maspin蛋白高表达率呈渐趋下降趋势,不典型增生与原位癌的表达无明显差异,浸润性乳腺癌表达最低,Logistic回归模型分析不同组织细胞核Maspin蛋白高表达间存在显著差异(P<0.05);而单纯性增生、不典型增生、原位癌和浸润性乳腺癌细胞浆Maspin蛋白高表达率分别为51.2%、85.0%、69.2%和63.5%,正常乳腺上皮未见细胞浆表达。不同组织Maspin蛋白的细胞浆表达同样有显著差异(P<0.01),其中癌旁不典型增生较乳腺癌有更高的细胞浆表达。进一步研究发现浸润性乳腺癌细胞核Maspin蛋白表达与乳腺?

Objective: To explore the significance of Maspin expression in breast cancer and lesions in breast tissues adjacent to breast cancers. Methods: S-P immunohistochemical method was used to assess the expression of Maspin in 104 invasive breast cancer, 26 breast cancer in situ, 43 cases of ductal epithelial simple hyperplasia and 20 cases of epithelial dysplasia in tissues adjacent to breast cancers and 10 normal tissues. The clinical pathological significance of Maspin expression was evaluated. And the relationship between expression of Maspin and that of ER, PR, c-erbB-2, CathD, VEGF in 104 cases of invasive breast cancer was statistically analyzed. Results: Positive immunoreactivity was mostly located in the nuclear of normal epithelium, strong positive nuclear expression was found in all the control cases (100%). Strong nuclear expression of Maspin in simple ductal epithelial hyperplasia, dysplasia, cancer in situ and invasive breast cancer was 76.7%, 65%, 69.2% and 49.0% respectively, which were all lower than that of control (P<0.05). There were significant difference in the expression of Maspin between cancers and lesions in the tissue adjacent to cancer. Immunohistochemical analysis showed that the Maspin plasma expression were detected in 63.5% of invasive breast cancers, 69.2% expression in cancer in situ and 85.0% expression in atypical heperplasia of tissues adjacent to cancers, 51.6% simple heperplasia of tissues adjacent to cancers, but nearly no expression in normal tissues. There were also significant difference in the Maspin plasm expression between cancers and tissues adjacent to cancers or nomal tissues(P<0.01). The results of analysis by Logistic regression showed that the nuclear expression of Maspin in invasive breast cancer was positively correlated with that of ER, while negetively correlated with the expression of c-erbB-2 and histological grading. No significant correlation were found between the nuclear expression of Maspin and that of PR, Cath-D, VEGF and lymph node metastasis. The plasma expression of Maspin in invasive breast cancer were not related to any pathological parameter. Conclusions: Overexpressions of Maspin protein in nuclear was mostly in simple hyperplasia adjacent to cancers or nomal tissues, meanwhile overexpression in plasma is relatively early event in mammary carcinogenesis. It maybe act an important role in the development of breast cancer.