α-黑素细胞刺激素在自体中厚移植皮片中的表达

Expression of α-melanocyte stimulating hormone in human split-thickness skin graft

目的检测α黑素细胞刺激素(αmelanocytestimulatinghormone,αMSH)在人体自体中厚移植皮片中的表达,并与自身正常皮肤作对照,初步认识αMSH在自体移植皮片过度色素沉着中的作用。方法利用免疫组织化学方法,检测人体自体中厚移植皮片与原供区及受区周围自体正常对照皮肤中αMSH的蛋白表达,并行统计学分析。结果αMSH的表达定位于表皮基底部黑素细胞、角朊细胞胞浆,在大部分自体中厚移植皮片中呈强阳性表达,表达率为611%,与自体正常对照皮肤中的表达差异有非常显著性意义(P<001);αMSH在受区与原供区周围正常对照皮肤中的表达差异无显著性意义。结论αMSH在自体中厚移植皮片中的表达较在自体正常对照皮肤中显著增高,在自体移植皮片过度色素沉着中起重要调控作用。

Objective\ To investigate the expression of α melanocyte\|stimulating hormone (α\|MSH) in human split\|thickness skin autograft and the role of α\|MSH in hyperpigmented process of the grafted skin. Methods\ Immunohistochemical technique was used to detect the expression and distribution of α\|MSH in the split\|thickness grafted skin and normal skin separately. Results\ The expression of α\|MSH in most of the split\|thickness grafted skin was much stronger than the control skin. The positive ratio of α\|MSH expression was 61\^1% in the split\|thickness grafted skin, 11\^1% in the normal skin of the donor area and 16\^7% in the normal skin around the recipient area.The expression of α\|MSH in the split\|thickness grafted skin was significant high, compared with the normal skins ( P <0 01). The expression of α\|MSH in the normal skin of the donor area was no statistic remarkably differences compared to the normal skin around the recipient area. Conclusion\ The results indicate that the expression of α\|MSH may markedly increase in the split\|thickness grafted skin and correlate with its pigmentation after the skin graft. Overexpression of α\|MSH may play an important role in hyperpigmented process of the skin graft.\;