8-溴-环腺苷酸对视网膜母细胞瘤细胞系端粒酶活性及动力学影响的研究

Effect of 8-bromum-cyclic adenosine monophosphate on the telomerase activity and dynamics in retinoblastoma cells

目的 探讨 8-溴 -环腺苷酸 (8- Br- c AMP)对视网膜母细胞瘤 (RB)细胞系端粒酶活性及细胞周期变化的影响。 方法 将培养的 RB细胞系分为对照组及实验组 (加 8- Br- c AMP培养组 ) ,分别进行体外培养 2 4、4 8、72 h后 ,以聚合酶链反应 -酶标免疫吸附实验方法检测其端粒酶活性 ,流式细胞仪检测细胞周期变化。 结果 各实验组 RB细胞端粒酶活性与对照组相比 ,差异均有显著性的意义 (P<0 .0 1)。8- Br-c AMP作用不同时间实验组的端粒酶活性吸光度 [A,旧称光密度 (OD) ]值与作用时间之间 ,呈显著负相关(r=- 0 .7789,F=33.936 ,P<0 .0 1)。实验组细胞周期变化 :G1 期比率上升 ,S期比率下降。 结论 8-Br- c AMP可减弱 RB细胞系端粒酶活性 ,影响其周期变化 ,抑制

Objective To investigate the effect of the 8-bromum-c yc lic adenosine monophosphate (8-Br-cAMP) on the telomerase activity and changes of cell cycle in retinoblastoma (RB) cells. Methods The cul tured RB cells were divided into the experimental group (8-Br-cAMP) and contro l group. After cultured for 24, 48 and 72 hours in vitro, the telomerase activit y of RB cells was detected by polymerase chain reaction enzyme-linked immunosor bent assay (PCR-ELISA) and the changes of cell cycle were detected by flow-cyt ometry. Results The difference of telomerase activity was sign ificant between the experimental groups and control group (P<0.01). There wa s a negative correlation between the A value of absorbance and the time in t he experimental groups (r=-0.778 9, F=33.936, P<0.01). The changes of the ce ll cycle were that the percentages increased in G 1 phase and decreased in S ph ases. Conclusion 8-Br-cAMP may weaken telomerase activity, a ffect the cell cycle, and inhibit the proliferation of RB cells.