缺氧对牛视网膜血管内皮细胞整合素受体α_vβ_3表达及功能的影响

Effect of hypoxia on expression and function of integrin rece ptor α_vβ_3 of bovine retinal v ascular endotheliocytes

目的 探讨缺氧对牛视网膜血管内皮细胞整合素受体αvβ3表达及功能的影响。 方法 以玻璃粘连蛋白 (Vn)包被培养皿 ,将牛视网膜血管内皮细胞置入正常及缺氧条件下 ,孵育 12、2 4、4 8、6 0、72 h后以酶联免疫吸附方法检测细胞表面整合素受体αvβ3的表达 ,并通过细胞粘附分析检测其功能。 结果 缺氧条件下 ,αvβ3的表达逐渐增加 ,在 4 8h达到顶峰 ,在 6 0、72 h仍较正常环境高。缺氧 2 4 h后 ,牛视网膜血管内皮细胞对细胞外基质 Vn的粘附明显增加。 结论 缺氧可上调培养的牛视网膜血管内皮细胞αvβ3的表达 。

Objective To investigate the effect of hypoxia on the e xp ression and function of integrin receptor α vβ 3 of bovine retinal vascular endotheliocytes. Methods Bovine retinal vascular endotheliocy tes in the culture dishes coated by vitronectin was put into the normal and hypo xemic condition, respectively. Enzyme linked immunosorbent assay and cel l-adhesion analysis were used to detect the expression and function of integrin receptor α vβ 3 in bovine retinal vascular endotheliocytes, respectively. Results Under the condition of hypoxia, the expression of α vβ 3 increased gradually, and reached the peak at the 48th hour. The expressio n of α vβ 3 at the 60th and 72nd hour in hypoxia group was higher than that in the normal group. Bovine retinal vascular endotheliocytes absorbed more Vn of extra-cellular matrixes (ECM) after cultured under hypoxemic condition for 24 hours. Conclusion Hypoxia may up-regulate the expression of α vβ 3, which promote the adsorbability of endotheliocytes.