人视网膜内界膜Ⅳ型胶原纤维的分子组成分析

Molecular composition of type Ⅳ collagenous fibres in internal limiting membrane of human retina

目的 分析人视网膜内界膜 型胶原纤维的分子组成。 方法 将手术中行内界膜剥除所取得的膜样组织经光学显微镜、电子显微镜鉴别确认为内界膜后 ,采用针对不同的 型胶原分子 (α1～α6 )的单克隆抗体 ,以免疫荧光法定位。 结果 人视网膜内界膜可为α3、α4、α5 型胶原分子免疫定位 ,α1、α2、α6型 型胶原分子则不能明确定位于人视网膜内界膜。 结论 人视网膜内界膜的 型胶原纤维由α3、α4、α5

Objective To analyze the molecular composition of type Ⅳ collagenous fibres in internal limiting membrane (ILM) of human retina. Methods ILM was surgically removed from retina and identified under p hase-contrast and transmission electron microscopes. Monoclonal antibodies aga inst different α chains (α1-α6) of type Ⅳ collagen were immuno-localized. Results α3, α4, and α5 chains of type Ⅳ collagen were imm uno-localized in human retinal ILM, while α1, α2, and α6 chains could not be immuno-localized. Conclusion Type Ⅳ collagenous fibres in h uman retinal ILM are composed of α3, α4, and α5 chains.