羊膜细胞培养液对体外培养视网膜组织中一氧化氮及合成酶的影响

Effects of culture medium of amniotic cells on NO and NOS in retina tissues in vitro

目的 观察羊膜细胞培养液对成年家兔体外培养视网膜组织的一氧化氮 (NO)及一氧化氮合成酶 (NOS)的影响 ,旨在为视网膜移植提供抗氧化的保护方法。 方法 健康成年家兔 30只 ,30只眼(取右眼 ) ,分成 3组 : (新鲜视网膜组 )、 (常规培养液组 ) , (羊膜细胞培养液组 ) ;将 、 组视网膜组织块放入相应培养基中培养 1周。 3组视网膜取材后进行 NO、NOS含量的测定。 结果 与 组相比 , 组 NO、NOS值显著性增高 (t=3.82 1、3.85 4 ,P<0 .0 0 1) ; 组与 组 NO值、NOS含量的差异比较无统计学意义 (t=1.6 5 7、1.74 5 ,P>0 .0 5 )。 结论 羊膜细胞培养液有清除自由基、增强抗氧化能力的作用。

Objective To observe the effects of culture medium of a mn iotic cells on NO and NOS in retinal tissues of rabbits in vitro in order to pro vide a protective method for antioxidation in retina transplantation. M ethods Thirty adult healthy rabbits (30 right eyes) were divided into 3 groups. Group Ⅰ: fresh retinal tissue; group Ⅱ: routine culture medium; group Ⅲ: culture medium of amniotic cells. The retinal tissues in group Ⅱ and Ⅲ we re cultured in the corresponding culture medium for 1 week. The content of NO an d NOS in retinal tissues in the 3 groups were determined. Results Compared with group I, the content of NO and NOS of group Ⅱ increased obvio usly (t=3.821, 3.854; P<0.001). There was no statistical difference of content of NO and NOS between group Ⅰ and Ⅲ (t=1.657, 1.745; P>0.05) . Conclusion Culture medium of amniotic cells may remove free r adicals and enhance the ability of antioxidation.