摘要
Objective:To investigate possible effects of nanophase powder of hydroxyapatite on proliferation of periodontal ligament cells. Methods: With sol-gel method, the nanophase hydroxyapatite powders were fabricated. These powders were proved nanopaticles by transmission electron microscope. The effects on proliferation of periodontal ligament cell(PDLC) were observed in vitro with MTT [3-(4,5dimethylthiazo;-2-yl)-2,5-diphenytetralium bromide] method. Results: On the 2nd,3rd,4th day after treated with nanoparticles of hydroxyapatite, the proliferate activity of the PDLC increases significantly, compared with those with dense hydroxyaoatite and control but no significant difference could be found between the dense hydroxyapatite and the control. Conclusion: Nanophase hydroxyapatite can promote the regeneration of periodontal tissue.
目的 :通过制备纳米羟基磷灰石 ,考察其对牙周膜细胞增殖和骨化细胞定向分化的诱导作用。方法 :采用溶胶 -凝胶法 ,在前驱体中加入鳌合剂柠檬酸 ,阻滞分子团聚。制作粉体后行扫描电镜、透射电镜、X线衍射分析和能谱分析。原代牙周膜细胞培养 ,第 4代细胞接种后 ,按1mg ml加入纳米羟基磷灰石粉体 ,以相等量的致密羟基磷灰石和空白作对照。作细胞形态和四甲基偶氮唑盐微量酶反应比色法分析。结果 :采用四水硝酸钙和磷酸三甲脂为前驱体 ,加入枸橼酸可制备纳米羟基磷灰石粉体 ,粒径约为 5 0nm。应用 2、3、4天纳米羟基磷灰石对牙周膜细胞增殖活性有明显促进作用 ,而致密羟基磷灰石与空白对照组差异无显著性。结论 :纳米羟基磷灰石有促进牙周膜细胞增殖作用 ,在牙周组织再生治疗中有一定的应用意义。
