Isolation and Evaluation of Nerve Growth Factor

神经生长因子的提取和鉴定(英文)

Objective: to obtain the high purified and active nerve growth factor (NGF) from mouse submaxillary glands. Methods: NGF was prepared from mouse submaxillary glands by the way of elution with CM 52 column. The molecular weight and purification of NGF were detected by SDS-PAGE polyacrylamide gel electrophoresis. The biological activity of NGF was verified thorough culturing DRG. Results: About 14 kDa stained band was observed on SDS-PAGE and it promoted proliferation of dorsal root gang lia (DRG). Conclusion: Good quality of NGF could be obtained with these methods.

目的 :制备具有生物学活性的神经生长因子(NGF)。方法 :以小鼠领下腺为原料 ,采用CM5 2多次洗脱的方法分离神经生长因子 ,SDS PAGE电泳检测NGF的分子质量和纯度 ,采用鸡胚背根神经节培养方法检测NGF的生物活性。结果 :经CM5 2多次洗脱的方法的NGF在SDS PAGE电泳显示为一条分子质量 14kDa的区带 ,生物学活性良好。结论 :本方法可用于NGF的制备。