HBs和HBx基因在HCC家庭聚集性中的作用研究

A RESEARCH IN THE EFFECT ON THE CLUSTERING OF HEPATOCELLULAR CARCINOMA IN FAMILIES BY THE HBs AND HBx GENES

目的 :探讨 HBs(S基因 )和 HBx基因 (X基因 )在原发性肝细胞癌 (HCC)家庭聚集性中的作用 ,为制定 HCC预防对策提供科学依据。方法 :应用配对原则在肝癌高发区选择肝癌高发家庭成员的同时 ,选择相同生活环境、生活习惯、生活条件和生活水平、同民族、同性别 ,年龄± 5岁的非癌家庭成员作为研究对象。采用 PCR技术和探针杂交法及免疫荧光显影技术对研究对象外周血清中 S和 X基因进行检测 ,应用配对χ2 检验进行统计分析。结果 :S基因在 HCC高发家庭成员组中的阳性率显著高于非癌家庭成员组 ;两组的男、女性及男女合并后的阳性率及分别为 6 0 .5 % (4 6 / 76 )、5 2 .9% (1 8/ 34)、5 8.2 % (6 4 / 1 1 0 )和1 5 .8% (1 2 / 76 )、2 9.4 % (1 0 / 34)、2 0 .0 % (2 2 / 1 1 0 ) (P <0 .0 0 5、 P <0 .0 5、 P <0 .0 5 )。 X基因在 HCC高发家庭成员组中的阳性率亦显著高于非癌家庭成员组 ,两组的男、女性及男女合并后的阳性率分别为 2 6 .3% (2 0 / 76 )、1 4 .7% (5 / 34)、2 2 .7% (2 5 /1 1 0 )和 2 .6 % (2 / 76 )、8.8% (3/ 34)、4 .5 % (5 / 1 1 0 ) (P <0 .0 0 5、 P >0 .0 5、 P <0 .0 0 5 )。结论 :HBV的 S基因和 X基因与 HCC的家庭聚集性存在密切关系。 S基因的检出率比 X基因检出率高 ,可作为

Objective:The clustering phenomenon of hepatocellular carcinoma (HCC) in the endemic area in Guangxi was investigated in the past few years. We found that the infection of hepatitis B virus (HBV) played an important role in the clustering of HCC in families.The significance of HBV S and X genes in the clustering of HCC in families is still unclear. The aim of the study is to probe the significance of the HBV S and X gene in the clustering of HCC, and to establish the possibility for the prevention of HCC.Methods:The subjects of the two groups:Group 1 had 2 or even more HCC cases in the family, and Group 2 hadn't any cancer cases in the family, were decided by matching methods,with the same living environment, living customs, living level and condition, the same sex and nationality, ages±5 years between the two groups. Sera were collected from the subjects, and the S and X genes were tested with polymerase chain reaction,to probe hybridization and immunofluorescence autoradiography. The paired chi square statistic analysis was applied to determine the different between the two groups.Result:There was significant difference between the two groups in the positive rate of S gene and X genes 58 2%(64/110),22 7%(25/110) and 20 0%(22/110),4 5%(5/110) (P<0 005,P<0 005).The positive rates of S gene in the male, female between the two groups were 60 5%(46/76),52 9%(18/34)and 15 8%(12/76),29 4%(10/34) respectively (P<0 005, P<0 05).The positive rates of X gene in the male, female between the two groups were 26 3%(20/76),14 7%(5/34) and 2 6%(2/76),8 8%(3/34) respectively (P<0 005, P>0 05) Conclusion:The study results show that there is a closely relationship between the HBV S gene, X gene and the clustering of HCC in families in the endemic area of Guangxi, China. The positive rate of S gene is higher than that of X gene, and the determination of S gene can be used as the mark reference of HBV infection. The effect on the occurrence of HCC by S and X genes is worth further investigating.