应用BSA系统检测隐孢子虫感染者外周血T细胞亚群及mIL-2R(英文)

DETECTION OF PBMC T CELL SUBSETS AND mIL-2R OF THE PERSONS INFECTED BY CRYPTOSPORIDIUM PARVUM BY USING THE SYSTEM OF BSA

目的 探讨隐孢子虫感染者机体细胞免疫功能的变化。 方法 采用生物素 链霉亲和素 (Biotin Streptavidin ,BSA)法对卵囊阳性患者分别进行外周血T细胞亚群、膜白介素 2受体 (mIL 2R)检测。 结果 隐孢子虫卵囊阳性者的CD3 + 、CD4+ 、CD8+百分率和CD4+ /CD8+ 比值分别为 ( 5 4.77± 7.3 3 ) %、( 3 8.17± 7.5 2 ) %、( 3 0 .64± 5 .87) %和 1.2 8± 0 .72 ,诱导期mIL 2R表达水平为 ( 3 2 .15± 2 .42 ) % ,两者分别与卵囊阴性者及静息期mIL 2R相比 ,差异有显著性 (P <0 .0 5～ 0 .0 1)。 结论 隐孢子虫感染者引起细胞免疫功能的变化 ,T细胞亚群、mIL 2R在抗隐孢子虫感染中起重要作用。

Objective To explore the changes of cellular immune function in the persons infected by Cryptosporidium parvum . Methods The system of biotin-streptavidin (BSA) was used to detect T cell subsets and membrane interleukin-2 receptor (mIL-2R) in peripheral blood mononuclear cell (PBMC) of the persons infected by C. parvum . Results The positive rates of CD3 + ,CD4 + ,CD8 + and CD4 + /CD8 + in the persons in whose stools C. parvum oocysts were found were (54.77±7.33)%,(38.17±7.52)%,(30.64±5.87)% and 1.28±0.72 respectively,and the level of mIL-2R in induction period was (32.15±2.42)%. It could be concluded that the positive rates of CD3 + ,CD4 + ,CD8 + and CD4 + /CD8 + in the persons in whose stools C. parvum oocysts were found were significantly different from those with no C. parvum oocysts were found ( P <0.05～0.01). The level of mIL-2R in induction period was significantly different from which in resting period ( P <0.05～0.01). Conclusion In the persons infected by C. parvum ,cellular immunity participates in resisting the parasite,and T cell subsets and mIL-2R play an important role.