雄性大鼠去势后基底前脑NOS及Nestin阳性神经元的变化

Changes in nitric oxide synthase immunoreactive and nestin immunoreactive neurons of the basal forebrain following castration of adult male rats

目的 探讨睾丸源性雄激素对基底前脑一氧化氮合酶 (NOS)及巢蛋白 (Nestin)阳性神经元的影响 ,为阐明基底前脑的功能提供资料。方法 将 16只成年健康雄性SD大鼠随机分为去势组和对照组 ,2周后用组织化学及免疫组织化学染色方法观察基底前脑的内侧隔核 (MS)、斜角带垂直支 (vDB)及水平支 (hDB)的NOS和Nestin阳性神经元的形态和数目变化。结果 与对照组相比较 ,去势组大鼠MS、vDB的NOS阳性神经元数分别明显升高了 37 2 %和 2 9 1% (P <0 0 5 ) ;而且去势组大鼠MS、vDB的Nestin阳性神经元数升高更明显 ,分别达 6 8 2 %和 5 6 9% (P <0 0 5 ) ,但去势组大鼠hDB的NOS和Nestin阳性神经元数均升高不明显 (P >0 0 5 )。去势组与对照组大鼠的NOS和Nestin阳性神经元的形态均相似。结论 雄性大鼠去势后可选择性地使基底前脑各亚区的NOS、Nestin阳性神经元数升高 ,但不影响神经元的形态 ,这可能与神经元的表达摆脱了睾丸源性雄激素的抑制作用有关。

Objective To explore the effects of androgen from testis on nitric oxide synthase(NOS) immunoreactive and nestin immunoreactive(IR) neurons in the basal forebrain of adult male rats. Methods Sixteen healthy adult male SD rats were randomly divided into control and castrated groups. After two weeks rats were sacrificed. Histochemical and immunohistochemical techniques were used to identify and compare the shapes and numbers of NOS-IR and Nestin-IR neurons in the medial septum(MS),vertical(vDB) and horizontal(hDB) limbs of the diagonal band of Broca between two groups.Results Compared with those in control group,the numbers of NOS-IR neurons in MS and vDB of castrated group significantly increased by 37.2% and 29.1%(P<0.05), respectively; the numbers of Nestin-IR neurons in the two subregions significantly increased by 68.2% and 56.9%(P<0.05),respectively, but the numbers of NOS-IR and Nestin-IR neurons in hDB did not increase to a significant level(P>0.05). The shapes of NOS-IR and Nestin-IR neurons in castrated group were similar to those in control group.Conclusion Castration of male rats selectively increases the numbers of NOS-IR and Nestin-IR neurons in the subregions of basal forebrain, and this may be related to the testis androgen inhibition of the expressions of both NOS and nestin.