白细胞介素-7选择性剪接变异体的研究

Expression of interleukin-7 mRNA and identification of its splice variants

目的 寻找人肿瘤细胞选择性的白细胞介素 7(Interleukin 7,IL 7)剪接变异体。方法 运用自行设计的IL 7引物 ,采用逆转录聚合酶链反应 (RT PCR)技术 ,分离正常肝组织、原发肝癌组织、正常胃组织和胃癌组织IL 7mRNA ,再对电泳所获条带进行克隆、测序。结果 正常肝组织、原发肝癌组织、正常胃组织和胃癌组织都能检测到IL 7mRNA ,而且从肝癌组织、胃癌组织中各分离出一条新带 ,经亚克隆及测序 ,证实这两条带分别是人IL 7基因cDNA第 4外显子 (肝癌组织 )和第 5外显子 (胃癌组织 )缺乏所致。结论 肝癌组织和胃癌组织存在不同选择性IL 7剪接变异体。这些变异体的发现 ,为肿瘤病人的免疫调节紊乱以及肿瘤病人的临床免疫治疗提供了新的研究方向。

Objective To investigate the expression of interleukin-7 in human liver and stomach, identify the splice variants of IL-7.Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used for investigating the expression of IL-7. According to IL-7 gene cDNA, the primers were designed and synthesized, then the splice variants of IL-7 in human cancer tissues was identify, cloned into vector and sequenced.Results the results showed that (1)the expression of IL-7 mRNA was nearly equal between human liver and stomach,(2) Two new bands of IL-7 was obtained from liver cancer and gastric carcinoma, one lacks exon 4, another exon 5.Conclusion IL-7 gene is expressed highly in both human liver and stomach . In addition, human liver cancer and gastric carcinoma can produce splicing variants of IL-7 by alternative splicing.