妊高征患者胎盘中转化生长因子β1表达及其与血清VCAM-1的相关性

The expression of transforming growth factor-β1 in pregnancy-induced hypertension and the relationship between the TGF-β1 and serum VCAM-1

目的 探讨胎盘中TGF - β1与血清血管细胞黏附分子 - 1(VCAM - 1)与妊高征发病的关系及两者的相关性。方法 被研究人群由 2 0例正常产妇和 4 0例不同程度的妊高征患者组成 ,胎盘组织由剖宫产术后取得 ,用免疫组化ABC染色法观察TGF - β1在胎盘中的分布与定位 ,通过高清晰度彩色病理图文分析系统对其进行定量分析 ,用酶联免疫吸附双抗体夹心法 (ELISA)测定血清VCAM - 1含量。结果 TGF - β1在各组胎盘合体滋养细胞层中均有不同程度的表达。中、重度妊高征组胎盘组织中TGF - β1的含量明显高于轻度妊高征组及正常组 (P<0 0 5 ) ;妊高征组血清VCAM - 1水平 (1310±ng/ml)比正常组 (6 0 9± 72ng/ml)升高显著 (P <0 0 1) ;胎盘组织中TGF - β1表达水平与血清VCAM - 1水平呈显著正相关 (P <0 0 1)。 结论 妊高征患者组织中TGF - β1明显升高 ,血清中VCAM - 1明显升高 ,且两者呈显著正相关 ,提示TGF - β1与VCAM -

Objective To study the expression of Transforming growth factor-β1(TGF-β1) in placental tissue of pregnancy-indeced hypertension(PIH) and the relationship between the level of TGF-β1 and the amount of vascular cell adhesion molecule-1(VCAM-1) in serum. Methods The study population consisted of 20 normal pregnancy women and 40 women with PIH. Placental tissues were obtained after caesarean section. Immunohistochemistry was used to observe the expression and distribution of TGF-β1 in placenta tissues. High resolution pathological image analysis system(HPIAS) was used to determine the expression of TGF-β1. VCAM-1 in serum was examined by enzyme linked immunoabsorbent assay(ELISA).Results TGF-β1 expressed in syncytiotrophoblast. The levels of TGF-β1 expression in placenta of moderate and severe PIH patients were significantly higher than that of normal group(P<0.05), Serum VCAM-1 was significantly lower than that of normal group(P<0.01). Significant positive correlation existed between the expression of TGF-β1 in placental tissues and the serum VCAM-1(r=0.969, P<0.01). Conclusion TGF-β1 expression increases in PIH and is positively correlated with serum VCAM-1, indicating their involvement in the pathogenesis of PIH.