Survivin在大肠癌中的表达及其与大肠癌增殖活性的相关分析

The Expression of Survivin and its Relationship with the Proliferative Activity in Colorectal Carcinoma

目的 :研究Survivin在大肠癌中的表达及其与大肠癌增殖活性的相关意义。方法 :应用免疫组化S P法检测 64例大肠癌组织及 1 0例癌旁组织的Survivin、增殖细胞核抗原 (PCNA)蛋白表达水平。结果 :癌旁大肠黏膜组织中均无Survivin表达 ,Survivin在大肠癌的表达阳性率为 53 % ,PCNA的增殖指数为 33 .65± 1 8.7%。Survivin蛋白表达与大肠癌的组织学类型、淋巴结转移相关 (P <0 .0 5 ,P <0 .0 5) ,而与浸润深度无关 (P >0 .0 5) ;Survivin表达与PCNA的阳性表达呈正相关。Survivin阳性组的PCNA的增殖指数为 53 .8± 1 8.6 % ,明显高于Survivin阴性组的 1 3 .5± 3 .5 % (P <0 .0 5)。结论 :Survivin在大肠癌中高表达 ,在大肠癌的发生、发展中起着重要的作用 ,与大肠癌的分化程度、淋巴结转移、高增殖有关。

Objective:To study the expression of survivin and its relationship with the proliferative activity colorectal carcinoma.Methods:Using immunohistochemistry (S-P method) to detect the protein expression of survivin and PCNA in colorectal carcinoma (n=64) and nearby no-tumorous tissues (n=10).Results:No expressed survivin was detected in no-tumorous tissues.The positive rates of survivin was 53%,the PCNA-LI was 33.65±18.7%.Significant relationships were found among survivin expression,histological type and lymph node metastasis (P<0.05,P<0.05),but not with the invasive depth (P>0.05).There was a positive correlation between the survivin and PCNA expression,the PCNA-LI in survivin-positive tumors was 53.8±18.6%,which was significantly higher than that in survivin-negative tumors (P<0.05).Conclusion:The expression of survivin is increased in colorectal carcinoma,which plays important role in the oncogenesis and progression of colorectal carcinoma and is related to histological type,lymph node metastasis and higher proliferation potential.Survivin can be a new diagnostic/therapeutic target to colorectal carcinoma.