PKC-α反义核酸对宫颈癌HeLa细胞增殖及细胞周期的影响

Effect of PKC-α asODN on proliferation and cell cycle in HeLa cells of cervical carcinoma/

目的 :探讨 PKC-α反义核酸对 He L a细胞增殖及细胞周期的影响。方法 :采用脂质体 (L P)介导法 ,用 0 .0 1～1.0 0μmol/L各浓度 PKC-α反义核酸 (as ODN)转染 He L a细胞 ;MTT法及软琼脂克隆形成法分别检测 He L a细胞生长指数 (growth index,GI)及其体外增殖能力 ;流式细胞术分析细胞周期。结果 :PKC- α as ODN0 .0 5～ 1.0 0 μmol/L 各浓度组 He L a细胞 GI显著降低 (P<0 .0 5 ) ,且呈量效依赖关系 ;其中 1.0 0μmol/L和 0 .5 0μm ol/L PKC-α as ODN对He L a细胞生长有非常显著抑制作用 (P<0 .0 1) ;0 .5 0 μmol/L PKC- α as ODN组 He L a细胞软琼脂克隆形成率均低于对照组 (P<0 .0 5 ) ;反义 PKC- α使细胞 G2 期百分比增高 (P<0 .0 1)。结论 :反义 PKC- α可通过阻滞细胞于 G2 期从而抑制 He L a细胞的生长。

Objective: To investigate the effect of antisense oligonucleotides of PKC-αon proliferation and cell cycle in HeLa cells of cervical carcinoma.Methods: PKC-α antisense oligonucleotides(asODN) and a random sequence as a control were transfected into HeLa cells by lipofectin(LP). The cell growth index (GI) and the clone formation rate of HeLa were detected by MTT and soft agar assay, respectively. Cell cycle was analyze by flow cytometry. Results: The GI of HeLa cells transfected by PKC-α asODN with concentration ranging from 0.05μm to 1.00μm was lower significantly than that of control groups(P<0.05), and there was a dose-dependent relationship among them. The inhibitive effects of 0.50μm and 1.00μm PKC-α asODN on the cell proliferation were strongest(P<0.01),and there was no significant difference between the two concentrations. After treated with antisense PKC-α, the percentage of cells in G 2 phase enhanced (P<0.01). Conclusion:Antisense PKC-αmay can inhibit cell growth in HeLa via hindering cell process in G 2 phase.