α——淀粉酶微胶囊化方法的研究

Investigation of the Method of Microencapsuhing α—1. 4-Glucan-4-Glucanohydrolase

膜材料为乙基纤维素,芯材为α—淀粉酶的水溶液,用高分子界面沉淀技术制备微胶囊。考察了乳化时的油水比、乙基纤维素浓度及表面活性剂等因素对微胶囊化的影响。在电子显微镜下观测,可以清晰地看到微胶囊壁的表面状态、膜内孔的结构和芯材在微胶囊内的分布。用重量法计算微胶囊制备的产率。将制备好的微胶囊在室温下干燥后粉碎,用水抽提出其中的α—淀粉酶,在60℃下测定酶活性。对于不同条件下制备的微胶囊,用酶活性的相对大小可定性地比较α—淀粉酶的包埋活性。

We prepared the microcapsule by use of precipitation of polymer on the interface. The film materical was Et-celullose, and the core materical was aqueous solution of α—1. 4-glucan-4-glucanohydrolase (EC 3. 2. 1. 1). We tested the effect of the ratio of oil-water volumes, the concentrations of Et-celullose and surfactants on the microencapsulation. Under an electron microscope, we could catch sight of the surface texture of the microcapsule wall, the construction of the pore through the film, and the room of the core in the film. The outputs were calculated by weighing of the microcapsule. After the dried microcapsule was broken, we extracted the EC 3. 2. 1. 1 with water from the fragments. The enzymatic activity was measured at 60℃. The enzymatic activity could show qualitativly the yield of the immobilized EC-3. 2. 1. 1 as the variety of conditions.