糖尿病视网膜病变中氧化应激反应的研究及应用

Studies and application of oxidative stress response in diabetic retinopathy

目的 通过研究抗氧化剂VitaminE在糖尿病动物模型 大鼠视网膜中对氧化应激反应的作用 ,探寻糖尿病视网膜病变的发病机理。方法 将大鼠随机分为正常组、糖尿病组、维生素E治疗 1组 (剂量 0 .12g·kg-1)、2组 (剂量 0 .2 5g·kg-1)。饲养 8个月后 ,观察其视网膜蛋白激酶C(proteinkinaseC ,PKC)的活性和无细胞血管及影细胞的变化。 结果 治疗 1组与糖尿病组大鼠视网膜PKC的活性及无细胞血管、影细胞无明显差异 (P>0 .0 5 ) ,治疗 2组与糖尿病组大鼠视网膜PKC的活性及无细胞血管、影细胞有明显差异(P <0 .0 5 )。结论 抗氧化剂VitaminE能抑制糖尿病视网膜病变的氧化应激反应进而减轻其早期病理损害 ,糖尿病视网膜病变的发病机理与氧化应激反应的关系有待于进一步被证实。

Objective To observe oxidative stress character is tics of antioxidant Vitamin E in diabetes rats,in order to investigate the path ogenic mechanism of diabetic retinopathy. Methods Diabetic rats and no diabetic rats that were fed standa rd diets or the diets supplemented with Vitamin E:group 1:0.12 g·kg -1 、 group 2:0.25g·kg -1 .Diabetes of at least 8 months to observe the protein k inase C activity and the change of accelular capillaries and pericyte ghosts. Results It's resulted in pericyte loss,accelular capillaries an d basement membrane thickening compared with diabetic controls.The development of acellular capillaries was inhibited markedly (P<0.05) in diabetic rats gr oup 2 that received supplemental vitamin E(0.25 g·kg -1 ) and the number o f pericyte ghosts tends to be reduced.Parameters of retinal oxidative stress,p rotein kinase C activity,and remained elevated, and those abnormalities were no rmalized by vitamin E therapy. But the group 1(Vitamin E:0.12 g·kg -1 ) did n't change markedly(P>0.05). Conclusion Antioxidants can inhibit the development of the earl y stages of diabetic retinopathy, and the mechanism by which this action occurs warrants futher investigation.