利多卡因对实验性哮喘豚鼠的作用及机制

The mechanism and effects of nebulized lidocaine on asthma guinea pigs

目的 观察雾化吸入利多卡因对实验性哮喘豚鼠的影响 ,并初步探讨其机制。方法 32只豚鼠随机分为四组 ,每组 8只 :正常组 (C组 )、哮喘组 (A组 )、地塞米松治疗组 (D组 )和利多卡因治疗组 (L组 )。采用卵蛋白致敏和激活制成豚鼠哮喘模型 ,观察各组的诱喘时间及血清和肺灌洗液中一氧化氮 (NO)水平的变化。结果 各治疗组诱喘时间均比哮喘组长。A组血清NO显著高于C组 (P <0 0 5 ) ;与A组相比 ,D组显著降低 (P <0 0 5 ) ,L组无明显差异。肺泡灌洗液中NO值A组较C组明显升高 (P <0 0 5 ) ;与A组比较 ,D组和L组均明显降低 (P <0 0 5 )。结论 雾化吸入利多卡因对实验性哮喘豚鼠有治疗作用 。

Objective To observe the effect of nebulized lidocaine on asthma guinea pigs and explore its mechanism. Methods Thirty-two guinea pigs were randomly assigned to one of four groups with 8 each. They were normal group (group C),asthma model group (group A),dexamethasone treatment group (group D),and lidocaine treatment group (group L). The guinea pigs were sensitized and challenged with ovalbumin (OA). Then the time needed for inducing asthma attack was observed and the concentration of nitric oxide in serum and bronchoalveolar lavage fluid was tested. Results Time needed for inducing asthma attack in group D and L was longer than that in group A. The concentration of nitric oxide in serum of group A was significantly higher than that in group C (P<0.05),and the value of NO in group D was significantly lower than that in group A (P<0.05),but there was no significant difference between group L and group A. The concentration of nitric oxide in bronchoalveolar lavage fluid in group A was significantly higher than that in group C (P<0.05),while the concentration in group D and group L was lower than that in group A (P<0.05). Conclusion Nebulized lidocaine has a therapeutic effect on guinea pigs asthma,and inhibition of the synthesis of nitric oxide of local tissue may be one of the mechanisms.