大肠癌患者围手术期血清IFN-γ和IL-4水平变化及意义

Dynamic study on serum levels of IFN-γ and IL-4 in perioperative period patients with colorectal cancer

目的探讨大肠癌患者围手术期血清干扰素-γ(IFN-γ)和白细胞介素-4(IL-4)水平的变化及其临床意义。方法采用ELISA法分别测定58例大肠癌患者手术前后血清IFN-γ和IL-4水平,并与健康对照组比较分析。结果大肠癌组治疗前IFN-γ水平降低(98.9±11.3pg/ml),而IL-4水平增高(128.3±14.8pg/ml),与对照组血清IFN-γ水平(157.5±17.0pg/ml)和IL-4水平(84.8±10.9pg/ml)比较均有显著性差异(P<0.01);且与淋巴结转移和肿瘤临床TNM分期有明显相关性(P<0.05)。行根治性手术组术后IFN-γ水平显著性增高(P<0.05),IL-4显著性降低(P<0.05);而姑息性手术组IFN-γ水平则无显著性增高(P>0.05),IL-4水平则无显著性降低(P>0.05)。结论大肠癌患者血清IFN-γ和IL-4水平的变化与肿瘤的浸润转移和病程有关,检测大肠癌患者血清IFN-γ和IL-4水平的变化,有助于估计患者的治疗效果和预后。

Objective To investigate the changes and clinical significance of the serum levels of Interferon-γ(IFN-γ) and Interleukin-4(IL-4) in the patients with colorectal cancer before and after operation. Methods The serum levels of IFN-γ and IL-4 in 58 patients with colorectal cancer were detected by ELISA method before and after operation and compared them with that of health controls. Results The serum levels of IFN-γ in patients with colorectal cancer before operation were significantly lower than that in control group (98.9±11.3pg/ml vs 157.5±17.0pg/ml, P<0.01), while the level of IL-4 was significantly higher (128.3±14.8pg/ml vs 84.8±10.9pg/ml, P<0.01). There was significant correlation between the levels with lymph node metastasis and clinical stage (Duckes). The levels of IFN-γ were significantly decreased in patients with colorectal cancer after radical resection (P<0.05), while the level of IL-4 was significantly decreased (P<0.05). But there was not significant changes of the levels of IFN-γ and IL-4 in patients with colorectal cancer after palliative operation (P>0.05). Conclusion The serum levels of IFN-γ and IL-4 are closely related to tumor invasion, metastasis and clinical stage (Duckes). Dynamic monitoring the levels of IFN-γ and IL-4 may be an important measures for evaluting invasion, metatasis, efficiency and prognosis for the patients with colorectal cancer.