不同环境小鼠的行为、记忆及脑突触蛋白磷酸化

COMPARISON OF BEHAVIOR,MEMORY AND SYNAPTIC PROTEIN PHOSPHORYLATION LEVELS OF MICE REARED IN DIFFERENT ENVIRONMENTS

本实验通过离体测定小鼠脑突触蛋白磷酸化水平探讨了丰富和单调环境饲养下小鼠行为、记忆差异的脑内突触化学机制。结果如下:(1)丰富环境小鼠小脑半球突触蛋白磷酸化水平的显著变化可能与小鼠对环境的适应有关;(2)丰富环境可增强记忆保持力;(3)两组小鼠行为训练后各脑区突触蛋白磷酸化水平的变化有差异,丰富环境组小鼠左、右海马测定值均升高。表明:不同环境小鼠行为、记忆的差异可能与环境对某些脑区突触蛋白磷酸化作用的影响有关。

The behavior, memory and synaptic protein phosphorylation of the main brain areas were compared between mice reared in enriched conditions (EC) and impoverished conditions (IC), and the changes of levels of synaptic protein phospohorylation in both untrained and trained mice were observed. Synaptosomes were prepared from mice reared in different types of environment and following training. The protein phosphorylation levels were detected by labelling with γ-^(32)P-ATP in vitro. The main results are as follows: (1) The alterations of protein phosphorylation of cerebellum of EC mice may be related to their adaptation to the complex environment; (2) Memory was facilitated by the environmental enrichment; (3) Levels of protein phosphoryation of EC's left and right hippocampi rose significantly through training. These data suggest that the different behaviors and memory of EC and IC mice may be related to the changes of protein phosphorylation of the relative brain areas, which are induced by different environments.