降低 ABC—ELISA 非特异性吸附的实验方法探讨

THE EXPERIMENTAL INVESTIGATION FOR DECREASE OF NON—SPECFICABSORBANCE IN ABC—ELISA

用生物素化的乙肝病毒表面抗原(BHZ-HBsAg)和亲和素辣根过氧化物酶复合物(ABC-HRP)作为检测系统,对降低 ABC-ELISA 非特异性吸附的方法作了探讨。结果表明.适当的 Avidin-HRP 稀释度、0.5%牛血清白蛋白封闭以及提高 ABC-HRP 稀释液的离子强度,均可有效降低 ABC-ELISA 的非特异性吸附。

The non—specific absorbance of the HRP complex is a severe interference in ABC ELISA.We searched for the available manners by which to decrease the non—specific noise using biotinized HBsAg and avidin—HRP complex as the detected system.It was shown that thecombination of appropriate dilution of the enzyme complex,of blockwith 0.5% bovine serum albumin and of the increase of ionic strength of the buffer solution is necessary for reduc- tion of the niose sigal.