PCR检测婴儿肝炎综合征中巨细胞病毒感染

DETECTION OF CYTOMEGALOVIRUS IN NEONATAL HEPATITIS SYNDROME BY POLYMERASE CHAIN REACTION

采用PCR技术对37例肝炎综合征患者进行了检测。结果表明,24例为HCMV阳性,占被检人数的64.9%,与已报导的资料相比,PCR方法的阳性检出率明显高于目前使用的其它方法.此外,灵敏性和特异性试验表明,HCMV—PCR方法足以能检测出0.1pg的HCMV的DNA,无非特异性带出现,因此,PCR技术具有高度敏感、特异性强、简单快速等优点,可以用于临床检测和诊断。

In order to investigate the infection status with human cytomegaloviru(HCMV),37 patients with neonatal hepatitis syndrome were analysed by polymerase chain reaction(PCR)The result revealed that 24 patients were positive fou HCMV,with an infection rate of 64.9%,which is higher than that obtained by other metheds.PCR can detect as less aso lpg HCMV-DNA.In ad- dititon,it is simple,rapid and specific.Therefore,PCR is a new powerful tool for detection of HCMV refection.