测定人血吲哚美辛的高效液相色谱法

HIGH—PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF INDOMATHACIN IN HUMAN SERUM

采用高效液相色谱法测定人血清中吲哚美辛浓度。色谱柱为SpherisorbC18(5μ,4.6×250mm),流动相为乙腈—水—冰乙酸(54.2V∶45.2V∶0.6V)。检测波长为258nm,用外标法峰高度定量(C=0.001 14H+0.00441,r=0.9993,线性范围:0.125～2.000μg/ml)。回收率为96.5%～97.1%,日内及日间变异系数均小于6%。本法适用于药代动力学和临床研究。

A high-performance liquid chromatographic method for the determination of indomethacin in human serum was devloped.The method utilizes a spherisorb C18 column,an absolute calibration curve(C=0.00114H+0.00441,r=0.9993),and ultraviolet detection,with reproducibility studies which yield coefficients of intraday and interday variation ranging from 1.32% to 5.56% and 4. 91% to 5.24%,respectively.The absolute recovery of indomethacin from serum with the concen- tration range of 0.125 to 1μg/ml was 96.5% to 97.1%.