摘要
用氟利昂去除法氏囊组织杂蛋白,并经甘油-酒石酸钾密度梯度离心进一步纯化,通过电镜观察、光密度测定和聚丙烯酰胺凝胶电泳(PAGE).证明得到的鸡传染性法氏囊病病毒(IBDV)形态完整。将纯化的IBDV 免疫 BALB/C 鼠,取其脾细胞与 SP2/0骨髓瘤细胞融合,用酶联免疫吸附试验(ELISA)和病毒中和试验(VNT)检测分泌抗体,获得了6株稳定分泌抗 IBDV 中和性单克隆抗体的杂交瘤细胞,分别命名为 NIB_1、NIB_2、NIB_3、NIB_4、NIB_5、NIB_6。还建立了检测 IBDV 的单克隆抗体双抗体 ELISA。
Purified IBDV was obtainecd from infected bursa of Fabricius by freon extraction and patassiumtartrate-glycerol density centrifugation to remove tissue proteins and other components.It was identified withelectron microscopy,optical density measurement and PAGE.Six stable hybridoma cell lines,secretingspecific neutralizing monoclonal antibodies to IBDV,were sereened by ELISA and VNT,and designated asNIB_1～NIB_6.Double-antibody sandwich ELISA was established to detect IBDV.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1993年第3期79-84,共6页
Journal of Nanjing Agricultural University
基金
高校博士学科点专项科研基金资助
关键词
鸡病
IBD
单克隆
抗体
infectious bursal discase virus(IBDV)
density gradient centrifugation
monoelonal antibody(McAb)
enzyme-linked immunosorbent assay(ELISA)
virus neutralizing test(VNT)
