TH蛋白在肾组织中的定位观察及其意义

Distribution of Tamm-Horsfall Protein in Rats Suffering from Gentamicin Nephrotoxicity and Masugi Nephrits

用过氧化物酶抗过氧化物酶(PAP)方法检查了大白鼠庆大霉素(GM)肾毒性模型、马杉肾炎和正常对照组肾组织中Tamm-Horsfall蛋白(THP)的分布情况。结果表明正常情况下THP为肾脏远端小管上皮细胞所排泌的一种糖蛋白,在肾小管受到损伤后,THP可沉积于间质内,这可能是由于尿液反流及外渗所致。间质THP沉积的多少似乎与小管损伤程度有关,而与炎症反应无关。因此,观察THP在间质分布情况似乎可作为判断肾小管损伤的一种形态学标志,并可用以估计肾脏疾病中肾小管的损伤程度。

The distribution of Tamm-Horsfall protein(THP)in kidneys was studied with per- oxidase-antiperoxidase reaction in rats suffering from gentamicin nephrotoxicity,mesugi nephritis and normal controls.(1)In rats with gentamicin nephrotxicity,the proximal tubules were destroyed seri- ously with disappearance of normal structure of the epithelial cells and tubules.THP deposits were seen in clumps or pieces of yellow-brown substances stained by PAP.(2)In masugi nephritis,the foot pro- cesses of podocytes were flattened and fused.The interstitium was infiltrated with inflammatory cells. THP deposits found in the interstitium were scanty.THP-stained casts were also seen in the tubules. The damage of the proximal and distal tubules was mild.(3)In control rsts,THP was located in the epithelial cells of the distal uriniferous tubules.It was suggested that THP might be a glycoprotein syn- thesized and secreted by the epithelial cells of ascending limb of loop of Henle and the distal convoluted tubules.The apearance of THP in the interstitium in damaged renal tubules might be caused by urinary intratubular backflow and extravasation.In addition,the amount of THP deposits in interstitum seemed to run in parallel with the degree of tubular damage apparently without relation to the reaction of inflammatory cells.The distribution of THP in the interstitium may serve as an index to assess the degree of tubular damage in renal diseases.