人乳吞噬细胞与三种细菌的相互作用

Interactions of Human Colostral Phagocytes with Staphylococcus Aureus,Escherichia Coli and Pseudomonas Aeruginosa

检测了人乳吞噬细胞对金黄色葡萄球菌、大肠埃希氏菌和铜绿假单胞菌的杀菌效果,并结合扫描电镜和透射电镜观察初乳吞噬细胞与三种细菌相互作用的形态变化。结果表明,母乳吞噬细胞具有相当的吞噬能力、且对大肠埃希氏菌和铜绿假胞菌在2小时内的持续杀菌效果明显优于对金黄色葡萄球菌。观察到母乳吞噬细胞胞浆中存在的大量脂肪空泡在吞噬杀菌过程中与吞噬溶酶体融合的现象,提示母乳吞噬细胞在母乳抗感染保护机制中的独特作用和意义。

The following experiments were carried out in vitro:1).the separate evalu- ation of phagocytosis and intracellular killing of unoposonized Staphylococcus aureus,Es- cherichis coli and Pseudomonos aeruginosa by human colostral phagocytes.2).the electron microscope observation(SEM amd TEM)on the morphological features during the process of phagocytosis and intracellular killing. The results show that phagocytosis of S.aureus,E.coli and P.aeruginosa by human colostral phagocytes was efficient(P<0.01).However,intracellular killing of E.coli and P. aeruginosa(P<0.01)was much better than that of S.aureus(P>0.05)within 2h.Under TEM,human colostral phagocytes displayed many lipid-filled vacuoles in their cytoplasm. The phenomena of microfilaments in colostral phagocytes, and fusion between some lipid- filled vacuoles and phagotysosoms/phagocytic vacuoles,and some unusual granules with electron density and crystalloid organization were also observed.