核素标记舌癌单抗导向肿瘤定位诊断的实验性研究

Radioimmunological localization study using 131-iodine radioalbelled monoclone antibody ASC5 against human tongue squamous-cell carcinoma

本文运用１２５Ｉ（１３１Ｉ）标记舌鳞癌细胞单抗（ＡＳＣ５ＭｃＡｂ）进行载瘤裸鼠体内实验，观察ＡＳＣ５单抗对舌癌定位效应及体内分布。探讨其临床应用前景。经降植烷处理ＢＡＬＢ／Ｃ小鼠腹腔注入ＡＳＣ５细胞产生腹水，纯化得ＩｇＧ纯品，以改良氯胺Ｔ法分别用１２５Ｉ和１３１Ｉ标记ＡＳＣ５ＭｃＡｂ和ＮＭＩｇＧ，在载瘤裸鼠尾静脉注入标记抗体作体内分布及显像研究。结果示：给药后７２小时肿瘤部位浓聚明显，９６小时肿瘤显像更为清晰，并持续到１６８小时，采用双核素减影术，可获良好肿瘤显像。Ｔ／ＮＴ≥２．９，单抗在载瘤裸鼠体内分布以脑为最低，而血液和肿瘤始终最高，７２小时肿瘤组织的ＩＤ／ｇ为９．２６±１．９６％，占第二位，与同型配对抗体ＮＭＩｇＧ相比具有显著性差异（Ｐ＜０．０５）。

Nude mice carrying human tongue squamous-cell carcinoma xenografts were studied in vivo.McAb ASC5 were purified from ascitic fluid obtained from BALB/c mice pristane-primed prior to being inocualted intraperitoneally with hybridoma cell, using with protein A sepharose CL-4B.Nude mice bearing xenografts were given i.v,injection of McAb labelled with 1311 and 1251.Mice were imaged daily with PHO gamma camera and sacrificed at interval after i.v.injection,the tumor and selected tissues were excised,weighted and counted.Gamma scanning indicated that there were a larger amount of activity detectable in tumor at 72h after i.v.injection 1311ASC5,then the tumor could be more clearly visualized at 96h and remained strongly position over the 7day period,with the background activity decreasing.Tumor to non-tumor ratio was more than 2.9.It was secondary best of the antibody uptake at 72h,approximately 9.20±1.96%ID/g(mean±SD),which compared with isotypical antibody-paired normal mouse IgG,the difference was significant at P<0.05.Results demonstrsted that ASC5 is useful in localizationdignosis and management of cancer.