DNA－DNA杂交技术在口腔链球菌鉴定中的应用

Applied DNA-DNA hybridization in determination of oral Streptococcus

定量ＤＮＡ－ＤＮＡ杂交技术是近年来发展起来的一种新的鉴定未知菌的手段，对于表型性状差别小，难以识别菌株的分类鉴定更有价值，对于解决种水平以上的分类学问题和新种的确定十分重要。本实验采用ＤＮＡ－ＤＮＡ分子杂交技术对４株临床表型特征大致相同的链球菌进行了鉴定，明确了细菌的分类。本文还就ＤＮＡ－ＤＮＡ杂交技术中的某些技术关键问题，包括ＤＮＡ的提取和精度，纯度要求，杂交的时间和温度以及ＤＮＡ同源率的判断标准进行了讨论。

Quantitative measurement of DNADNA hybridization is a new technique for determination of unknown bacteria.It is more valuable how to identify strains which is rare difference on phenotypic characteristics.It is important to resolve bactericial taxonomy and identify new strain.Four strains of Streptococcus are determinated by hybridization of DNA.Some technique problems are discussed.Such as purification,precision of DNA and temperature as well as time of DNA hybridization and standard of judgement.