rIL-6抗癌机理初探(Ⅱ)-rIL-6对小鼠脾脏NK细胞和CTL细胞体内外调节效应

Effect of Recombinant Human Interleukin-6 on Marine NK and CTL Cells in vitro and in vivo

为了探讨rIL-6对NK和CTL细胞的调节效应,我们进行了rIL-6对小鼠脾脏NK细胞和CTL细胞功能影响的体内外实验研究。实验采用C57BL/6荷瘤或正常小鼠,给予rIL-6或生理盐水,连续10天,第15天取小鼠脾脏进行NK细胞或CTL细胞的活性测定。结果表明:(1)rIL-6在体外对NK细胞在小剂量时有促进作用,在大剂量时有抑制作用;对CTL细胞则表现出随IL-6剂量的增加,促进作用明显增强;(2)rIL-6对正常鼠体内NK细胞杀伤活性有促进作用;(3)对荷瘤鼠体内NK细胞和CTL细胞杀伤活性均有明显的促进作用;(4)rIL-6对荷瘤所致NK功能下降具有预防作用。提示rIL-6对小鼠NK细胞和CTL细胞杀伤活性的促进效应可能是rIL-6发挥抗癌作用的一个途径。

A study on the effect of rlL-6 on murine NK and CTL cells in vitro and in vivo was taken. The normal C57BL/6 mice and the EL4 carrying mice were injected with rIL-6 (10μg / day, i. p. tid) or NS respectively for 10 day and at 15th day, the spleen was treated to single cell suspension for assay of NK and CTL. The results showed that (1) the NK activity was improved by 6.25~100U / ml rIL-6, the optimal dose of IL-6 was l2.5U/ml. The CTL activity was improved by 50~200U/ml IL-6 . The optimal dose was 200U / ml IL-6 .(2) IL-6 may improve the NK function within normal mice and NK and CTL function within tumour earring mice models through promoting their cytotoxicity. (3) although tumour earring decreased the NK'S function, the IL-6 could regulated the immunological function to antagonize the immunological inhibitions.