干扰素-α对LTBMC中慢性粒细胞白血病细胞作用的体外研究

In Vitro Study of the Effects of Interferon-α on Chronic Myelogenous Leukemia Cells in LTBMC

通过体外骨髓长期液体培养(LTBMC)，我们研究了IFN-α对CML细胞的作用。在LTBMCk ,于培养开始加一次IFN-α(10^2，10^3,10^4U／ml)，对非贴壁层的细胞数和基质细胞层的形成无明显影响．但大剂量IFN-α（10^3U和10^4U／ml组)对非贴壁层CFU-GM抑制明显；每周连续加用IFN-α（10，10^2，10^3U／ml)，对非贴壁层细胞数和CFU-GM抑制明显，而且INF-α浓度愈高．贴壁层的形成愈少。联合INF-α和LTBMC可使部分病人非贴壁层Ph(+)染色体提前消失或Ph(-)比例提高。上述结果表明IFN-α，优先抑制晚期CFu-GM，对基质细胞也有抑制作用，LTBMC联合INF-α对Ph(+)CML细胞有协向净化作用。

The effects of interferon-α (IFN-α) on chronic myelogenous leukemia cells were studied in vitro by long-term bone marrow culture(LTBMC). There were no inhibition of cellularity from non-adherent layers and formation of adherent layers, however, CFU-GM from non-adherent layers was inhibited at IFN-α 103U/ml and 104 U/ml groups, when IFN-αwas added only at initiation of culture. If IFN-α was continuously added weekly, the cellularity and CFU-GM of non-adherent layers were significantly inhibited, and the formation of adherent layers was inversely associated with the increasing concentration of IFN-α. in addition, Ph(+) cells in non-adherent layers were disappeared early and the percentage of Ph(-) population was increased with the combination of IFN-α and LTBMC. It is concluded that IFN-αselectively inhibits the later CFU-GM of CML cells and the development of stromal cells, the combination of IFN-αand LTBMC might exert a synergically purging effect on Ph(+) CML cells