IL-2基因疗法对骨髓移植后免疫及造血重建的影响

Effect of IL-2 Gene Therapy on Immune and Hematopoietic Reconstitution after Bone Marrow Transplantation

本文以大剂量化疗后骨髓功能严重损伤做为实验模型,动态观察了成纤维细胞介导的IL-2基因疗法对同基因骨髓移植后免疫及造血功能重建的影响。结果表明,IL-2基因疗法能显著增强实验小鼠骨髓细胞的NK活性、LAK活性及ConA刺激的增殖反应,但对骨髓CFU-GM,CFU-MK、CFU-E集落形成无影响,提示成纤维细胞介导的IL-2基因疗法能加快和增强骨髓移植后的免疫功能重建过程,提高骨髓移植抗肿瘤作用,并可减少骨髓移植后感染等并发症。该结果为IL-2基因疗法应用于骨髓移植提供了一定的实验依据。

In the present study, the effect of fibroblast-mediated IL-2 gene therapy on immune and hematopoietic reconstitution was observed after syngeneic bone marrow transplantation (BMT) in the mice which had received high-dose chemotherapy. The NK activity , LAK activity and the proliferation of BMT were augmented significantly , while there were no effects on the formation of CFU-GM, CFU-MK and CFU-E of bone marrow after IL-2 gene therapy. The results suggested that fibroblast-mediated IL-2 gene therapy can accelerate and prompt the process of immune reconstitution, then enchance the antitumor effect and reduce the complication such as infection after BMT. The experiment provide basis for the application of IL-2 gene therapy in BMT in the future.