阿片肽对小鼠脾单个核细胞趋化活性的增强作用

The Spleen Mononuclear Cells of Mice Chemotaxis is Enhanced by Opioid Peptides

应用体外细胞培养、Ｂｏｙｄｅｎ小室检测方法，研究阿片肽对小鼠脾单个核细胞趋化活性的作用。结果表明：阿片肽可明显增强小鼠脾单个核细胞趋化活性，作用浓度为１０－１１～１０－５ｍｇ／ｍｌ，峰值为１０－７ｍｇ／ｍｌ；其作用可被阿片肽受体阻断剂纳络酮阻断。由此推断，阿片肽对小鼠脾单个核细胞趋化活性的增强作用是通过阿片肽受体介导而完成的。

The opiate peptides β-END and M-ENK have a broad spectrum of biologic activies including lymphocyte proliferation, natural killer cell activity and antibody production. The current study demonstrated that β-END and M-ENK could enhance mouse spleen mononuclear cell chemotaxis. The response to both β-END and M-ENK were observed in the range 10-11～10-5 mg/ml,with peak activities occuring at 10-7mg/ml.The effect could be block by naloxone.This result suggested that enhanced chemotaxis was mediated via an opiate receptor.