人参茎叶皂甙对创伤小鼠活化T细胞内cAMP代谢、蛋白激酶A活性的影响

Effect of Ginsenoside on cAMP Metabolism, Protein Kinase A Activityin Activated T cells from Traumatized Mice

皮下注射人参茎叶皂甙（ＧＳ）５０ｍｇ／ｋｇ，连续４天，可明显拮抗撞击创伤小鼠活化Ｔ细胞内ｃＡＭＰ含量、腺苷酸环化酶（ＡＣ）、蛋白激酶Ａ（ＰＫＡ）活性的升高及ｃＡＭＰ－磷酸二酯酶（ｃＡＭＰ－ＰＤＥ）活性的降低。０．１～１００ ｇ／ｍｌ的ＧＳ在体外也可降低创伤小鼠活化Ｔ细胞内ｃＡＭＰ含量及ＡＣ和ＰＫＡ的活性，并升高ｃＡＭＰ－ＰＤＥ活性。表明ＧＳ对创伤后Ｔ细胞功能受抑状态的纠正作用与此有关。

ubcutaneous administration of ginsenoside (GS) at dosage of 50mg/kg 4d, could resist the elevation of cAMPcontent, adenylate cyclase (AC), protein kinase A (PKA) activity and the reduction of cAMP-phosphodiesterase(cAMP-PDE) activity in activated T cells from traumatized mice. GS ranging from 0. 1 to 100 g/ml could in vitroreduce cAMP content, AC and PKA activity, while increase cAMP-PDE activity in activated T cells from traumatizedmice. It is inferred that the corrective effect of GS on the suppression of T cells function after trauma is related to it.