血清GGT及其高分子形式定量检测的临床意义

CLINICAL SIGNIFICANCE OF QUANTITATIVE INVESTIGATION OF SERUM GAMMA-GLUTAMYL TRANSFERASEAND ITS HIGH-MOLECULAR FORM

对68例原发性肝癌、136例肝外胆道梗阻和其他肝病、以及30名健康献血员,采用聚丙烯酰胺凝胶梯度电泳分离高分了γ-谷氨酰移换酶区带(HM-GGT),并进行定量检测。结果表明各肝胆疾病组GGT总活力及HM-GGT均有不同程度升高(肝外胆道梗阻》原发性肝癌》肝内胆汁郁积≥急、慢性肝炎≥肝硬化)。资料显示:(1)GGT升高是肝胆受累的灵敏指标,GGT正常几可除外肝病;(2)GGT>350u/L,HM-GGT>35u/L,H/T≥30%一般仅见于肝外胆道梗阻,有别于肝内胆汁郁积及其他肝病。但无助于肝外良恶性胆道梗阻的鉴别。

With vertical slab electrophoresis on polyacrylamide gradient gel, serum high-molecular form of gamma-glutamyl transferase (HM-GGT) was fractionated and quantitatively measured in 68 patients with primary hepatocellular carcinoma (PHC), 136 patients with extrahepatic cholestasis and other liver diseases, and 30 healthy controls. The results showed that different increases of serum GGT and HM-GGT were seen in a variety of hepato-biliary disorders (extrahepatic cholestases >>PHC>>intrahepatic cholestascs ≥aucte or chronic viral hepatitis ≥ liver cirrhosis). In conclusion: (1) Increase of GGT was a sensitive index about hepato-biliary disorders. Hepatosis would hardly exist without increase of GGT. (2) Generally, only in the patients with extrahepatic cholestases were GGT≥150u/L, HM-GGT≥35u/L and H/T≥30% founded, which were beneficial to the differential diagnosis of intra and extrahepatic cholestasis, but not helpful to the distinction between the benign and malignant extrahepatie obstruction.