试管母种高效快速扩接试验

An Experiment of Inoculating Test-tube Maternal Strains

进行试管母种扩接时 ,首先经无菌操作加入适量的无菌水 ,再用灭菌后的接种铲将试管母种的菌丝体捣碎 ,制成“液体菌种”进行扩接。该法操作简便 ,接种速度快 ,不仅成品率高 ,而且菌龄齐。

At the beginning of this test-tube strains inoculation,put into the tube adequate amount of aseptic water and mash the mycelia of cultured strains with a sterilized inoculator.Therefore, liquid inoculant is made for the transplantation into other prepared culture media.This convenient method can effectively produce strong hyphae of same age and height with high survival rate.