PCNA、P53和BCL-2在非小细胞肺癌中的表达及临床意义

Expression and Its significance of PCNA, P53 and BCL-2 Protein in Non-small-cell Lung Cancer

目的 研究PCNA、P5 3和BCL - 2三种基因蛋白与非小细胞肺癌 (NSCLC)临床病理特征的关系。方法 应用S -P免疫组化法检测 4 4例手术切除NSCLC标本中三种基因蛋白的表达。结果 4 4例标本中 2 9例 (6 5 .91% )PCNA过度表达 ,2 3例 (5 2 .2 7% )P5 3过度表达 ,2 1例 (47.73% )BCL - 2过度表达。PCNA和P5 3阳性表达率随分化程度升高而升高。BCL - 2阳性表达率随分化程度降低而降低。且高、低分化组间均有统计学意义 (P≤ 0 .0 5 )。PCNA阳性表达率在肺癌淋巴结有无转移组间有统计学意义 (P≤ 0 .0 5 )。结论 PCNA、P5 3和BCL - 2基因过度表达可能与NSCLC的发生、发展有关。

Objective To study the relationship between the expression of PCNA, P53,BCL-2 protein and the clinical pathological characteristics of non-small-cell lung cancer (NSCLC).Methods Expression of PCNA, P53 and BCL-2 protein was detected in 44 NSCLC samples by immunohistochemistry. Results The positive rate of PCNA, P53 and BCL-2 protein was 29 (65.91%), 23 (52.27%) and 21 (47.73%) respectively. The positive rate of PCNA and P53 in higher differentiation group was significantly lower than that in lower differentiation group (P≤0.05). The positive rate of BCL-2 in higher differentiation group was significantly higher than that in lower differentiation group (P≤0.05). The difference of the positive rate of the expression of PCNA protein between the group of lymph node metastasis and the group of non-lymph node metastasis were significant (P≤0.05). Conclusions Overexpression of PCNA, P53 and BCL-2 genes may play an important role in the oncogenesis and development of NSCLC.