摘要
目的 :合成和表征O 羧甲基 N 半乳糖化壳聚糖衍生物作为潜在的肝靶向基因载体。方法 :以天然聚合物壳聚糖为原料 ,首先制备得O 羧甲基壳聚糖 ,然后在其 2 NH2 上和乳糖酸反应 ,制得O 羧甲基 N 乳糖酰化壳聚糖 ;或与乳糖反应 ,用KBH4还原 ,制得O 羧甲基 N 乳糖胺化壳聚糖。结果与结论 :分别用FT IR、1HNMR、13 CNMR和元素分析对其进行了表征。用粉末X 衍射、DSC、TG对其物理性质进行了分析。制得的O 羧甲基 N 乳糖酰化壳聚糖O 羧甲基 N 乳糖胺化壳聚糖有望作为潜在的肝靶向基因载体。
AIM:To synthesize and to characterize O -carboxymethyl- N -galactosylated chitosan derivatives for potential gene delivery carriers for hepatocyte-targeting.METHOD: Firstly,a water-soluble O -carboxymehyl chitosan(CMCS) was prepared.To give galactosylated O -carboxymethyl Chitosan(Gal-CMCS),lactobionic acid was added and galactose group was introduced into the amino groups of CMCS Lactosaminated O -carboxymethyl chitosan(Lac-CMCS) was prepared by reaction of CMCS and α-lactose,followed by reaction with KBH 4 RESULT and CONCLUSION: FTIR, 1 H NMR , 13 C NMR and elemental analysis characterized the chemical structures of O -carboxymethyl galactosylated chitosan derivatives Some physical behaviors were analyzed by X -ray diffraction(WXRD) and differential scanning calorimetry (DSC) The O -carboxymethyl- Ν -galactosylated chitosan derivatives may be used as potential gene delivery carriers for hepatocyte-targeting
出处
《中国天然药物》
SCIE
CAS
CSCD
2004年第6期354-358,共5页
基金
国家 973项目资助 (No G19990 64 70 5 )~~