幽门螺杆菌感染小鼠胃模型的研究

Study on HP infection mouse model which mimics human gastric disease

①目的 探索建立幽门螺杆菌感染昆明小鼠的胃感染模型的方法。②方法 采用国际标准菌株NCTC11637灌喂昆明小鼠,所有动物灌喂前用酒精预处理一次,再隔天灌喂,共5次,每次0.4mL。实验组茵液灌喂,对照组生理盐水灌喂。所有动物灌喂前禁食、禁水12小时,灌喂后再禁食水4小时。在末次灌喂后2、4、6、8周分批以脊椎脱臼法处死实验组动物,分别用细菌学及组织学方法评价HP感染及其引起的胃组织炎症反应。③结果 造模2、4、6、8周后,动物细菌培养阳性率分别是75％、100％、60％、30％;对照组是0％。胃黏膜快速尿素酶试验阳性率分别是50％、56％、90％、70％;对照组38％。HP感染4周后胃组织甲苯胺蓝染色胃黏膜表面及胃小凹内可见数量不等的HP,实验组与对照组的HP感染定植量的差异有显著性;HE染色可见轻度的炎性反应,但与对照组相比差异无显著性。④结论 用灌喂HP的方法能建立早期的小鼠感染模型。

Objective To establish a model of KM mice infected with Hp. Method HP(NCTC11637)and NS were orally inoculated to 40 mice of experimental group and 10 mice of control group respectively. At 2,4,6,8 weeks after inoculation, stomach tissues of experimental animal were taken out for the examination of bacteriology and pathology, evaluated for inflammation and HP colonization. Result The positive rate of germiculture were 75% ,100% ,60% ,30% in experimental animal at 2,4,6,8 weeks,that of the control group was 0%. The positive rate of rapid urease test of stomach mucous membrane were 50% ,56% ,90% ,70% in experimental group. At 4 weeks after inoculation,there was a large number of Hp in the mucous surface and in the top of the gastric pit with higher density. Tissue sections showed low - grade gastric inflammation. The histological changes were similar to those of human with HP infection. Conclusion The early infection model of KM mouse has been established by orally inoculation.