固定化脂质体色谱研究中药复方的细胞膜通透性成分及其质量控制

Analysis of Biomembrane Permeable Compounds and Quality Control in Combined Prescription of Traditional Chinese Medicine by Immobilized Liposome Chromatography

提出了固定化脂质体色谱(immobilizedliposomechromatography,ILC)研究中药复方的细胞膜通透性成分及其质量控制的方法。以当归补血汤及其组分为例,考察了它们在ILC色谱柱上的分离效果;当归补血汤水提取液和75%乙醇提取液在ILC色谱柱上各有8个保留峰,当归水提取液、甲醇提取液和75%乙醇提取液在ILC色谱柱上分别有3、6、9个保留峰,黄芪水提取液、甲醇提取液和75%乙醇提取液在ILC色谱柱上各有7个保留峰;建立了当归补血汤及其组分中阿魏酸和蒿本内酯的定量分析方法,当归补血汤水提取液和75%乙醇提取液中阿魏酸的含量分别为0.0743%和0.0688%,蒿本内酯的含量分别为0.0472%和0.457%,当归水提取液和75%乙醇提取液中阿魏酸的含量分别为00694%和0.0691%,蒿本内酯的含量分别为00781%和0.455%。

A new method of immobilized liposome chromatography (ILC) was proposed to analyse the biomembrane permeable compounds in combined prescription of traditional Chinese medicine. Separation for the extracts of combined prescription of Danggui Buxue decoction and its compositions with different extract processes on ILC column was compared, it was found that there are respectively 8 peaks with significant retention on ILC for water extract and 75% ethanol extract of combined prescription of Danggui Buxue decoction, and 3,6 and 9 peaks respectively for the water extract, methanol extract and 75% ethanol extract of Rdix Anelica Snensis, and 7 peaks respectively for the water extract, methanol extract and 75% ethanol extract of radix astragli. A quality control method was also developedbased on the technique, which was able to simultaneously determine the concentrations of ferulic acid and liguistilide in extracts of combined prescription of Dnggui Buxue Decoction and Angelica sinensis by water and 75% ethanol, and its result show that the concentrations of ferulic acid in water extract and 75% ethanol extract of combined prescription of Danggui Buxue decoction is respectively 0.0743% and 0.0688%, and liguistilide, 0.0472% and 0.457%; the concentrations of ferulic acid in water extract and 75% ethanol extract of Angelica sinensis is respectively 0.0694% and 0.0691%,and liguistilide, 0.0781% and 0.455%.