肉苁蓉总苷对D-半乳糖脑老化模型小鼠的保护作用

Protective effects of gloycosides of cistanche on the D-galactose induced brain aging model mice

目的 探讨肉苁蓉总苷(GCs)对D 半乳糖(D gal)脑老化模型小鼠的保护作用机制。方法实验分 30d和 50d,每期NIH小鼠各为 120只,随机分为 6组:正常对照组 (NC)、模型组 (D gal)、VitaminE组、GCs组:分小、中、大剂量组[ (mg.kg-1.d-1 )31. 25、62. 5、125],每组 20只。除NC组外,在各组小鼠皮下注射D gal(50mg.kg-1.d-1 );NC组皮下注射NS, D gal组和NC组给予等容积饮水灌胃,VitaminE组和GCs组灌胃给药,每日 1次,均连续皮下注射和灌胃 30d和 50d。分别测定 5min错误次数、潜伏期、脑组织SOD的活性、MDA、LF含量,并观察 50d后海马CA1区组织结构形态的变化。结果 (1)5min内的错误次数: 30d和 50d的D gal组分别为(8. 25±4. 03)次和(9. 63±6. 18)次,均显著高于其它各组(P均 <0. 01)。(2)潜伏期: 30d和 50d的D gal组分别为 (25. 21±13. 86)s和(31. 75±17. 77)s,低于NC组、GCs中剂量组和大剂量组(P<0. 05,P<0. 01)。(3)SOD活性: 30d和 50dD gal组脑组织的SOD活性较其它各组低(P<0. 05,P<0. 01)。(4)MDA含量: 30d和 50dD gal组脑组织的MDA含量均较其它各组高 (P<0. 05,P<0. 01)。(5)LF含量: 30d和 50dD gal组脑组织的LF含量均较NC组高, 30d的脑组织GCs小剂量组、VitaminE组及 50dGCs各组及VitaminE组均较D

Objective To observe the influence of glycosides of cistanche (GCs) on the D-galactose induced brain aging model mice and explore the mechanism of GCs.Methods There were two stages including 30 and 50 days. 120 NIH mice of every stage were randomly divided into six groups: normal control group (NC), model group (D-gal), VitaminE group , GCs groups which was divided into lower dose, middle dose and large dose[(mg.kg -1d 1) 31.25, 62.5, 125]. Every group had 20 mice. Except for NC group, other groups were given hypodermic injection of D-gal (50mg.kg -1.d -1). NC group was given hypodermic injection of NS. D-gal group and NC group were given oral administration of water.VitaminE group and GCs groups were given oral administration. Mice of various groups of every stage were repeatedly given hypodermic injection and oral administration one time every day , and respectively measured erroneous number in 5 minutes , latent period and SOD activities,MDA content and LF content (brain ) after 30 and 50 days. Results (1) Compared with other groups, D-gal groups were obviously higher on erroneous number in 5 minutes which were 8.25±4.03 of 30 days and 9.63± 6.18 of 50 days(P<0.01).(2) Compared with NC groups, middle dose and large dose groups of GCs, D-gal groups were lower on latent periods which were (25.21±13.86)s of 30 days and (31.75±17.77)s of 50 days (P<0.05 and P<0.01).(3) Compared with other groups, D-gal groups of brain tissue were lower on SOD activities of 30 days and 50 days. (P<0.05 and P<0.01). (4) Compared with other groups, D-gal groups of brain were higher on MDA contents of 30 and 50 days. (P<0.05 and P<0.01). (5)Compared with NC groups, D-gal groups were higher on LF contents of brain tissue of 30 and 50 days. Compared with D-gal groups, lower dose group of GCs and VitaminE group of 30 days, and various groups of GCs and VitaminE group of 50 days were lower on LF contents of brain tissue (P<0.05 and P<0.01).(6)D-gal group was different from NC group ,large dose group of GCs and VitaminiE group on hippocampus CA1 region. of 50 days.ConclusionsIt indicated that GCs could improve the function of learning and memory ,increase SOD activities and decrease MDA and LF contents of brain, the mechanism of which may relate with antioxidation.