大穗黑麦草成熟种子愈伤组织诱导及分化的研究

Studies on Callus Induction and Differentiation of Mature Seed of Lolium grandispicum Y·J·Fei

以大穗黑麦草成熟种子为外植体 ,采用MS基本培养基 ,研究了不同浓度的 2 ,4-D和 6-BA及其组合对其愈伤组织诱导的影响 ,不同浓度的NAA和 6-BA组合对愈伤组织分化成苗的影响。结果表明 :不同浓度 2 ,4-D对大穗黑麦草愈伤组织诱导率差异显著 ,以 7mg/L效果最好 ,浓度过高 ,愈伤组织诱导率下降 ;在 0～ 1 .0mg/L范围内 ,6-BA浓度越高 ,愈伤组织诱导率越高 ,但长势渐差 ;2 ,4-D + 6-BA的不同配比浓度的愈伤组织诱导率存在差异 ,以MS + 7mg/L 2 ,4-D + 0mg/L 6-BA的组合为最优 ,产生的愈伤组织数量多、质量好 ;NAA+ 6-BA不同配比浓度的愈伤组织分化率存在一定差异 ,以MS +NAA 0 .5mg/L + 6-BA 1mg/L的愈伤组织分化率最高。

Taking the mature seed of Lolium grandspicum Y·J· Fei as explant and adopting MS basal medium,the influence on callus inducing by different concd.of 2,4-D,6-BA and their combination,as well as the influence on callus differentiation by NAA and 6-BA combination were studied.The results showed that different concd.2,4-D have significant variation on callus inducing ratio and the concd.of 7 mg/L is most effective.While with the increase of concd.the callus inducing ratio decrease.In 0-1.0 mg/L,the higher the 6-BA concd.is,the higher the callus inducing ratio is, but the weaker the growth is.Different concd.of 2,4-D+6-BA combination have variation on callus inducing and the combination of MS+7 mg/L 2,4-D+0 mg/L 6-BA is the optimum,which produce most callus with high quality.Different concd.of NAA+6-BA combination have variation on callus differentiation,with the highest differentiation on MS+NAA 0.5 mg/L+6-BA 1 mg /L.A callus inducing and differentiation system is established by taking the mature seed of Lolium grandispicum Y·J· Fei as explant.