微阵列技术筛选肺鳞癌分子标志物的研究

Screening of molecular markers of lung squamous cell carcinoma by microarrays

目的 研究肺鳞癌基因表达谱 ,筛选肺鳞癌的差异表达基因。方法 利用cDNA微阵列技术研究 6个分期为Ⅰ期的手术切除鳞癌标本的基因表达谱 ,找出在 6个基因表达谱中均呈上调或下调的基因 ,并选择其中表达上调的nm2 3与表达下调的BRCA2基因 ,利用免疫组化方法进行验证。结果 共有 10 7个基因在全部 6个肺鳞癌标本中出现差异表达 ,其中 2 6个表达上调 ,81个表达下调。nm2 3蛋白在 2 0个肺鳞癌标本中表达增强 ,而BRCA2则呈低表达 ,与cDNA微阵列技术检测结果相符。结论 cDNA微阵列技术可用于肺鳞癌差异表达基因的研究 ,利用多张微阵列芯片重复筛选出的差异表达基因可初步作为肺鳞癌的候选分子标志物。

Objective To identify molecular markers of lung squamous cell carcinoma by cDNA microarray technique. Methods cDNA expression profiles were examined by microarrays of 6 surgical specimens of stage Ⅰ lung squamous cell carcinomas. Those genes, either up-regulated or down-regulated in every specimen studied, were identified. The expression levels of nm23 and BRCA2 by the squamous cell carcinoma of the lung were further examined by immunhistochemical techniques. Results A total of 107 genes were identified, of which 26 were up-regulated and 81 were down-regulated in all six specimens. Immunhistochemical staining showed that, compared with normal lung tissues, the intensity of nm23 expression by the squamous cell carcinoma of lung was significantly increased while that of BRCA-2 was decreased. Conclusion cDNA microarrays can be used to identify gene expression profile of lung cancer, some of which may be used as markers of lung squamous cell carcinoma.