胰腺癌胆囊收缩素受体蛋白表达的研究

Expression of cholecystokinin receptor protein in human pancreatic cancer.

目的 探讨胆囊收缩素受体在正常胰腺组织、胰腺癌组织、胰腺癌细胞株SW1990中的表 达,以明确胆囊收缩素受体与胰腺癌的关系。方法 应用免疫组织化学法和免疫细胞化学法分别检测 正常胰腺组织、胰腺癌组织、胰腺癌细胞株SW1990中胆囊收缩素受体A、B亚型的表达。结果在正常 胰腺组织中缺乏胆囊收缩素受体A、B亚型的表达;在胰腺癌组织、人胰腺癌细胞株SW1990中也不表达 胆囊收缩素受体A亚型,但可见胆囊收缩素受体B亚型的表达;胆囊收缩素受体在癌细胞株SW1990中 的表达主要位于细胞膜上,细胞质内也可见。结论 胆囊收缩素受体B在胰腺癌组织、腺癌细胞株 SW1990中表达;胆囊收缩素受体B亚型主要位于细胞膜上;胆囊收缩素受体B亚型在胰腺癌细胞生长 中可能发挥重要作用。

Objective To investigate the expression of CCK receptor protein in normal pancreatic tissue and pancreatic cancer cell line SW1990. Methods ABC immunohistochemistry staining and immunocytochemistry staining were used to detect the CCK receptor protein expression in normal pancreatic tissue and pancreatic cancer tissue and pancreatic cell line. Results Expression of cholecystokinin receptor B type protein was detected in human pancreatic cancer cell line SW1990, but absent in normal pancreas and pancrectic cancer tissues. Expression of cek receptor A type was absent in all cells and tissues. CCK receptor was observed both in cell membrane and cytoplasm. Conclusions Cholecystokinin receptor B type was localized on cell membrane and cytoplasm, and was a new expression receptor in pancreatic cancer cell line SW1990. Cholecystokinin receptor may play an important role in the growth of pancreatic cancer. Furthermore, use of CCK inhibitor may be of significance for the prevention and treatment of pancreatic cancer.