玉米不同品种(系)抗感粗缩病(RBSDV)与3种防御酶同工酶关系的研究

Research on the relationship between maize resistance to RBSDV and defense isoenzymes

本实验是在完成湖北省玉米粗缩病病原分子生物学鉴定的基础上,根据60多份玉米材料田间自然抗病性鉴定结果,从中选择具有代表性的3个不同抗感病性品种,从生理生化角度研究了玉米品种抗感粗缩病与寄主防御酶POD、EST及PPO同工酶的关系。结果表明,不同抗感性玉米品种POD同工酶的表达具有显著差异,既存在可能与寄主抗性相关的同工酶组成性表达,又具有受病毒侵染后的诱导性表达;EST同工酶种类的表达与品种的抗感病性没有相关性,只是在表达活性上有一定的差异;PPO同工酶受病毒侵染后诱导表达的差异与品种生育期具有一定的相关性;玉米不同生育期其POD、EST、PPO同工酶在在一定的变化。

Based on the previous study on the molecular identification of Rice black-streaked dwarf virus (RBSDV) , this research was performed for further understanding of the relationship between resistance of maize to RBSDV and the expression of defense-related isoenzymes. Three representative maize varieties with different resistant level to RBSDV were selected from more than 60 varieties in field. The investigation showed that peroxidase (POD) isoenzymes expressed in obviously different patterns in the resistant and susceptible varieties. Both constitutive and virus-inducible expression of POD were found in all tested varieties,in which the constitutive expression might contribute to the host resistance. Expression of esterase (EST) isoenzymes varied in different developmental stages, but showed the same pattern in the resistant and susceptible varieties. Polyphenol oxidase (PPO) isoenzymes were stimulated by the virus infection and the enzyme expression also varied with the maize developmental stages. These results suggest that isoenzyme expression of POD, EST and PPO is developmentally regulated and varied with the maize varieties and virus infection.