能量条件对鸭下丘脑5-羟色胺样神经元的影响

Effect of Energy on Serotonin-like Neurons in Duck Hypothalamus

选用来自同一父母代的1日龄健康樱桃谷鸭60羽,饲喂1周后,随机分为2组:高能组和低能组,分别饲喂高能和低能日粮。饲喂4周后,处理动物:灌流固定,取全脑后固定4h,入8%蔗糖溶液中4℃保存。制作石蜡切片,采用免疫组化链霉亲和素-生物素-过氧化物酶复合物法(Streptavidin-biotin-peroxidasecomplexmethod,SABC)进行染色,并用图像分析系统作半定量分析。结果显示:高能组室旁核(17.73±1.41个/19.46×103μm2)及腹内侧核(15.80±1.71个/19.46×103μm2)有大量的阳性胞体;低能组外侧区(11.25±1.53个/19.46×103μm2)也有大量的阳性胞体,而室旁核没有。上述研究结果表明,下丘脑5-羟色胺样神经元参与摄食和能量代谢的调节。

In the present study, the immunohistochemical method streptavidin-biotin-peroxidase complex (SABC) was used to examine the effect of energy on serotonin-like neurons in hypothalamus of Cherry valley ducks bred with high energy feed and low energy feed, respectively. All films were analysed by using a computer-assisted image analysis system. In high energy group, labeled cells are widely distributed in paraventricular nucleus(PVN,17.73±1.41 neurons/19.46×103μm2) and ventromedial hypothalamus (VMH, 15.80±1.71 neurons/19.46×103μm2). In low energy group, labeled cells are widely distributed in lateral hypothalamus(LH, 11.25±1.53 neurons/ 19.46×103μm2), yet none in PVN.These results indicate that serotonin-like neurons in hypothalamus are involved in the regulation of food intake and energy metabolism.