羟乙葛根素对大鼠局灶性脑缺血再灌注损伤细胞凋亡及p53表达的影响

Effect of hydroxyethylpuerarin on apoptosis and expression of p53 in focal brain ischemia reperfusion injury rats

目的观察异黄酮类羟乙葛根素在大鼠局灶性脑缺血再灌注损伤时的抗凋亡作用。方法利用大鼠大脑中动脉栓线阻断法制备局灶性脑缺血再灌注损伤模型 ,利用病理学检查、免疫组织化学方法观察羟乙葛根素对缺血再灌注大鼠的脑组织细胞凋亡及p5 3表达的影响。结果羟乙葛根素能改善局灶性脑缺血再灌注损伤的病理学改变 ,减轻细胞凋亡的程度 ,抑制p5 3的表达。结论羟乙葛根素对大鼠局灶性脑缺血再灌注损伤具有保护作用 ,其作用机制可能与羟乙葛根素抗细胞凋亡 ,抑制p5 3表达有关。

PurposeHydroxyethylpuerarin(compound N-2035) is modified in structure from puerarin, a kind of isoflavone that was extracted from Chinese traditional medicine. This study is to investigate the protective effect of hydroxyethylpuerarin on focal brain ischemia-reperfusion injury in rats.MethodsThe models of focal brain ischemia reperfusion injury by middle cerebral artery occlusion (MCAO) established in Wister rats were used for HE stain, TUNEL and determination of p53.ResultsHydroxyethylpuerarin can significantly improve the pathological changes and inhibit apoptosis in hippocampus CA1 area and at the same time decrease the expression of p53.ConclusionHydroxyethylpuerarin can relieve brain damage induced by focal ischemia/reperfusion in rats, which may be related to the decrease of the expression of p53 and the inhibition of apoptosis.