白叶枯病菌毒素对水稻根冠细胞微丝分布的影响

Effect of Pyricularia Oryzae toxins on distribution of microfilment in rice root cap cells

用白叶枯病菌株Ah28产生的毒素,处理水稻野败型细胞质雄性不育系珍汕97A,以异硫氰酸荧光素-鬼笔环肽为探针,观察了毒素处理前后微丝骨架的形态及分布变化。结果显示,毒素处理后,珍汕97A的根冠细胞微丝的密集分布被破坏,纤丝状微丝不复存在,微丝集聚成粗束,有的呈碎片状。说明毒素影响微丝的分布,并探讨了毒素致病机理及微丝参与信号转导的可能性。

The distribution of actin filaments(AFs) in the root cap cells of rice Zhenshan97A was studied by using fluorescence probe FITC-Phalloidin.When the root cap cells were treated by toxins from Xanthomonas oryzae AH28,the distribution pattern of AFs changed into bundles or granules.Those suggested that the microfilaments was destroyed by toxins.